ARG81510
Human HMW Kininogen ELISA Kit
Human HMW Kininogen ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81510-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81510-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81510-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81510-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81510-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81510-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81510-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81510-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81510-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81510-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81510-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81510 Human HMW Kininogen ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HMW Kininogen in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | HMW Kininogen |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8% Inter-Assay CV: 6.4% |
| Alternate Names | Williams-Fitzgerald-Flaujeac factor; Kallidin II; High molecular weight kininogen; KNG; Fitzgerald factor; Alpha-2-thiol proteinase inhibitor; BDK; BK; Kininogen-1; HMWK; Kallidin I; Ile-Ser-Bradykinin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KNG1 |
| Gene Full Name | kininogen 1 |
| Background | This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014] |
| Function | (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. [UniProt] |
| Highlight | Related products: HMW Kininogen antibodies; HMW Kininogen ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Bradykinin is released from kininogen by plasma kallikrein. Hydroxylation of Pro-383 occurs prior to the release of bradykinin. Phosphorylated by FAM20C in the extracellular medium. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81510 Human HMW Kininogen ELISA Kit User's manual |
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