ARG81772
Human Heme Oxygenase 1 ELISA Kit
Human Heme Oxygenase 1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81772-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81772-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81772-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81772-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81772-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81772-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81772-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81772-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81772-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81772-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81772-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81772 Human Heme Oxygenase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Heme Oxygenase 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Heme Oxygenase 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.85 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2% |
| Alternate Names | bK286B10; Heme oxygenase 1; HO-1; EC 1.14.99.3; HMOX1D; HSP32 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HMOX1 |
| Gene Full Name | heme oxygenase 1 |
| Background | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008] |
| Function | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. [UniProt] |
| Highlight | Related products: Heme Oxygenase 1 antibodies; Heme Oxygenase 1 ELISA Kits; Heme Oxygenase 1 Duos / Panels; Related news: Keap1-Nrf2-ARE antibody panel is launched New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG81772 Human Heme Oxygenase 1 ELISA Kit User's manual |
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