ARG82607

Human Hsp 27 ELISA Kit

Human Hsp 27 ELISA Kit for ELISA and Human

Overview

Product Description ARG82607 Human Hsp 27 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Hsp 27 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity This kit could assay both natural and recombinant Human Hsp 27.

No significant cross-reactivity or interference was observed in the following samples:
Human: IFN gamma, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL17A, IL18, IL21, IL22, IL23, MCP1, TGF beta 1, TNF alpha and VEGF.
Mouse: IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha.
Rat: IFN gamma, IL1 beta, IL4, IL6, IL10 and TNF alpha.
Target Name Hsp 27
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.7 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.3 - 2000 pg/ml
Sample Volume 10 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 4.0%
Alternate Names HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28

Application Instructions

Assay Time ~ 2.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3315 Human HSPB1

Swiss-port # P04792 Human Heat shock protein beta-1

Gene Symbol HSPB1
Gene Full Name heat shock 27kDa protein 1
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]
Function Involved in stress resistance and actin organization. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. [UniProt]
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PTM Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925). [UniProt]
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