ARG81347
Human HtrA1 ELISA Kit
Human HtrA1 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG81347 Human HtrA1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HtrA1 in serum, placenta and cell culture supernatant. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | The assay recognizes HtrA1 full length (50 kDa) and the truncated form of HtrA1 (aa. 141-480, 37 kDa). It does not cross-react with human HtrA3 or HtrA4. |
| Target Name | HtrA1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 391 pg/ml |
| Sample Type | Serum, placenta and cell culture supernatant. |
| Standard Range | 0.39 - 25 ng/ml |
| Sample Volume | 100 µl |
| Alternate Names | CARASIL; L56; HtrA; ARMD7; High-temperature requirement A serine peptidase 1; PRSS11; ORF480; EC 3.4.21.-; Serine protease 11; Serine protease HTRA1 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | Liquid |
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| Storage Instruction | Store components at 4°C or -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | HTRA1 |
| Gene Full Name | HtrA serine peptidase 1 |
| Background | This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008] |
| Function | Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. [UniProt] |
| Highlight | Related products: HtrA1 antibodies; HtrA1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
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| ARG81347 Human HtrA1 ELISA Kit User's manual |
 Download
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