ARG82698

Human IDS / Iduronate 2 Sulfatase ELISA Kit

Human IDS / Iduronate 2 Sulfatase ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82698-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82698-002 Standard 2 X 10 ng/vial 4°C
ARG82698-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82698-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82698-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82698-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82698-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82698-008 25X Wash buffer 20 ml 4°C
ARG82698-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82698-010 STOP solution 10 ml (Ready to use) 4°C
ARG82698-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82698 Human IDS / Iduronate 2 Sulfatase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IDS / Iduronate 2 Sulfatase in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name IDS / Iduronate 2 Sulfatase
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.6%
Alternate Names Alpha-L-iduronate sulfate sulfatase; Iduronate 2-sulfatase; Idursulfase; SIDS; EC 3.1.6.13; MPS2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3423 Human IDS

Swiss-port # P22304 Human Iduronate 2-sulfatase

Gene Symbol IDS
Gene Full Name iduronate 2-sulfatase
Background The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]
Function Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. [UniProt]
Cellular Localization Lysosome. [UniProt]
Highlight Related products:
Iduronate 2 Sulfatase antibodies; Iduronate 2 Sulfatase ELISA Kits;
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Simplify the ELISA analysis by GainData
PTM The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. [UniProt]
Title Download Link
ARG82698 Human IDS / Iduronate 2 Sulfatase ELISA kit User manual download icon Download