ARG80116

Human IFN gamma (High sensitive) ELISA Kit

Human IFN gamma (High sensitive) ELISA Kit for ELISA and Human

Cancer kit; Developmental Biology kit; Immune System kit; Signaling Transduction kit

Component

Cat No Component Name Package Temp
ARG80116-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80116-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80116-003 Standard diluent buffer 20 ml 4°C
ARG80116-004 Antibody conjugate concentrate 400 µl 4°C
ARG80116-005 Antibody diluent buffer 16 ml 4°C
ARG80116-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80116-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80116-008 20X Wash buffer 50 ml 4°C
ARG80116-009 TMB substrate 12ml 4°C (Protect from light)
ARG80116-010 STOP solution 12ml 4°C
ARG80116-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80116 Human IFN gamma (High sensitive) ELISA Kit is a high sensitive Enzyme Immunoassay kit for the quantification of Human IFN gamma in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Canine IFN- gamma;cotton rat IFN- gamma;equine IFN- gamma;feline IFN- gamma;mouse IFN- gamma;porcine IFN- gamma;rat IFN- gamma
Target Name IFN gamma
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.95 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 1.95 - 125 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IFNG
Gene Full Name interferon, gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
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Research Area Cancer kit; Developmental Biology kit; Immune System kit; Signaling Transduction kit
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Title Download Link
ARG80116 Human IFN gamma (High sensitive) ELISA Kit User manual download icon Download