ARG70035
Human IFN gamma recombinant protein (Active) (His-tagged, C-ter)
Human IFN gamma recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IFN gamma recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IFN gamma recombinant protein |
| Species | Human |
| A.A. Sequence | MQDPY VKEAE NLKKY FNAGH SDVAD NGTLF LGILK NWKEE SDRKI MQSQI VSFYF KLFKN FKDDQ SIQKS VETIK EDMNV KFFNS NKKKR DDFEK LTNYS VTDLN VQRKA IHELI QVMAE LSPAA KTGKR KRSQM LFQGR RASQ with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is < 1.1 ng/ml. |
| Alternate Names | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNG |
|---|---|
| Gene Full Name | interferon, gamma |
| Background | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015] |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| PTM | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
