ARG70130
Human IGF1 recombinant protein (Active) (His-tagged, C-ter)
Human IGF1 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IGF1 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IGF1 |
| Species | Human |
| A.A. Sequence | MGPET LCGAE LVDAL QFVCG DRGFY FNKPT GYGSS SRRAP QTGIV DECCF RSCDL RRLEM YCAPL KPAKS A with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce MCF-7 cells proliferation. The ED50 for this effect is 0.9-3.1 ng/ml. The specific activity of recombinant human IGF-I is approximately > 1.2 x 10^3 IU/mg. |
| Alternate Names | MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IGF1 |
|---|---|
| Gene Full Name | insulin-like growth factor 1 (somatomedin C) |
| Background | The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015] |
| Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
