ARG82798

Human IGFBP1 ELISA Kit

Human IGFBP1 ELISA Kit for ELISA and Human

Overview

Product Description ARG82798 Human IGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of IGFBP1 in Human serum.
Tested Reactivity Hu
Tested Application ELISA
Specificity Cross-Reactivity: Not react with IGFBP2, 3, 4 and 5.
Target Name IGFBP1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.5 µg/L
Sample Type Serum
Standard Range 1 - 250 µg/L
Sample Volume 25 µl
Precision Intra-Assay CV: 2.8%
Inter-Assay CV: 6.2%
Alternate Names IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1

Application Instructions

Assay Time 1 hour 15 min

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3484 Human IGFBP1

Swiss-port # P08833 Human Insulin-like growth factor-binding protein 1

Gene Symbol IGFBP1
Gene Full Name insulin-like growth factor binding protein 1
Background This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients. [provided by RefSeq, Aug 2017]
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt]
Cellular Localization Secreted. [UniProt]
PTM Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. [UniProt]
Title Download Link
ARG82798 Human IGFBP1 ELISA Kit User's manual download icon Download