ARG81411

Human IGFBP4 ELISA Kit

Human IGFBP4 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81411-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81411-002 Standard 2 X 32 ng/vial 4°C
ARG81411-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81411-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81411-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81411-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81411-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81411-008 25X Wash buffer 20 ml 4°C
ARG81411-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81411-010 STOP solution 10 ml (Ready to use) 4°C
ARG81411-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81411 Human IGFBP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IGFBP4 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IGFBP4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 250 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 500 - 32000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%
Inter-Assay CV: 6.9%
Alternate Names IBP4; IBP-4; IGFBP-4; Insulin-like growth factor-binding protein 4; HT29-IGFBP; IGF-binding protein 4; BP-4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3487 Human IGFBP4

Swiss-port # P22692 Human Insulin-like growth factor-binding protein 4

Gene Symbol IGFBP4
Gene Full Name insulin-like growth factor binding protein 4
Background This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProt]
Highlight Related products:
IGFBP antibodies; IGFBP ELISA Kits; IGFBP Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81411 Human IGFBP4 ELISA Kit User's manual download icon Download