ARG81412
Human IL1 Receptor I ELISA Kit
Human IL1 Receptor I ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81412-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81412-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81412-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81412-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81412-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81412-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81412-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81412-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81412-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81412-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81412-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81412 Human IL1 Receptor I ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL1 Receptor I in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL1 Receptor I |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 62.5 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 125 - 8000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 6.2% |
| Alternate Names | Interleukin-1 receptor alpha; sIL-1R1; p80; CD121A; mIL-1RI; IL1R; P80; Interleukin-1 receptor type I; IL1RA; D2S1473; Interleukin-1 receptor type 1; IL-1R-alpha; IL-1RT-1; CD121 antigen-like family member A; IL-1R-1; sIL-1RI; IL-1RT1; mIL-1R1; CD antigen CD121a |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL1R1 |
| Gene Full Name | interleukin 1 receptor, type I |
| Background | This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013] |
| Function | Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. [UniProt] |
| Highlight | Related products: IL1 Receptor antibodies; IL1 Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding). Rapidly phosphorylated on Tyr-496 in response to IL-1, which creates a SH2 binding site for the PI 3-kinase regulatory subunit PIK3R1. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81412 Human IL1 Receptor I ELISA Kit User's manual |
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