ARG70054
Human IL11 recombinant protein (Active) (His-tagged, N-ter)
Human IL11 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human IL11 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL11 |
| Species | Human |
| A.A. Sequence | PGPPP GPPRV SPDPR AELDS TVLLT RSLLA DTRQL AAQLR DKFPA DGDHN LDSLP TLAMS AGALG ALQLP GVLTR LRADL LSYLR HVQWL RRAGG SSLKT LEPEL GTLQA RLDRL LRRLQ LLMSR LALPQ PPPDP PAPPL APPSS AWGGI RAAHA ILGGL HLTLD WAVRG LLLLK TRL with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce T11 cells proliferation. The ED50 for this effect is < 0.2 ng/ml. The specific activity of recombinant human IL-11 is approximately > 1 x 10^7 IU/mg. |
| Alternate Names | Oprelvekin; Interleukin-11; AGIF; Adipogenesis inhibitory factor; IL-11 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL11 |
|---|---|
| Gene Full Name | interleukin 11 |
| Background | The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
| Function | Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
