ARG70055
Human IL12A / IL12 p35 recombinant protein (Active) (His-tagged, C-ter)
Human IL12A / IL12 p35 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL12A / IL12 p35 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL12A / IL12 p35 |
| Species | Human |
| A.A. Sequence | MRNLP VATPD PGMFP CLHHS QNLLR AVSNM LQKAR QTLEF YPCTS EEIDH EDITK DKTST VEACL PLELT KNESC LNSRE TSFIT NGSCL ASRKT SFMMA LCLSS IYEDL KMYQV EFKTM NAKLL MDPKR QIFLD QNMLA VIDEL MQALN FNSET VPQKS SLEEP DFYKT KIKLC ILLHA FRIRA VTIDR VMSYL NAS with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED50 for this effect is < 49 pg/ml. |
| Alternate Names | CLMF; Cytotoxic lymphocyte maturation factor 35 kDa subunit; NFSK; NK cell stimulatory factor chain 1; CLMF p35; NKSF1; IL-12 subunit p35; IL-12A; Interleukin-12 subunit alpha; P35 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL12A |
|---|---|
| Gene Full Name | interleukin 12A |
| Background | This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
