ARG70057
Human IL13 recombinant protein (Active) (His-tagged, C-ter)
Human IL13 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL13 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL13 |
| Species | Human |
| A.A. Sequence | MSAPF SFLSN VKYNF MRIIK YEFIL NDALN QSIIR ANDQY LTAAA LHNLD EAVKF DMSAP FSFLS NVKYN FMRII KYEFI LNDAL NQSII RANDQ YLTAA ALHNL DEAVK FDFWE THGTK NYFTS VAHPN LFIAT KQDYW VCLAG GPPSI TDFQI LENQA with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.8 ng/ml. The specific activity of recombinant human IL-13 is approximately > 1 x 10^6 IU/mg. |
| Alternate Names | Interleukin-13; P600; IL-13 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL13 |
|---|---|
| Gene Full Name | interleukin 13 |
| Background | This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008] |
| Function | Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
