ARG70058

Human IL15 recombinant protein (Active) (His-tagged, N-ter)

Human IL15 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (N-ter) Active Human IL15 recombinant protein
Tested Application SDS-PAGE
Target Name IL15
Species Human
A.A. Sequence NWVNV ISDLK KIEDL IQSMH IDATL YTESD VHPSC KVTAM KCFLL ELQVI SLESG DASIH DTVEN LIILA NNSLS SNGNV TESGC KECEE LEEKN IKEFL QSFVH IVQMF INTSL E with polyhistidine tag at the N-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce NK cells proliferation. The ED50 for this effect is < 3 pg/ml. The specific activity of recombinant human IL-15 is approximately > 5 x 10^9 IU/mg.
Alternate Names IL-15; Interleukin-15

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL15
Gene Full Name interleukin 15
Background The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]
Function Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. [UniProt]
Cellular Localization Isoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. [UniProt]