ARG82622

Human IL16 ELISA Kit

Human IL16 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82622-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82622-002 Standard 2 X 10 ng/vial 4°C
ARG82622-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82622-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82622-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82622-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82622-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82622-008 25X Wash buffer 20 ml 4°C
ARG82622-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82622-010 STOP solution 10 ml (Ready to use) 4°C
ARG82622-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82622 Human IL16 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL16 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL16
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.3%
Inter-Assay CV: 6.1%
Alternate Names prIL-16; Lymphocyte chemoattractant factor; PRIL16; IL-16; NIL16; LCF; Pro-interleukin-16

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3603 Human IL16

Swiss-port # Q14005 Human Pro-interleukin-16

Gene Symbol IL16
Gene Full Name interleukin 16
Background The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Function Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.

Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. [UniProt]
Cellular Localization Interleukin-16: Secreted. Isoform 1: Cytoplasm. Isoform 3: Cytoplasm. Nucleus. [UniProt]
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IL16 antibodies; IL16 ELISA Kits; IL16 Duos / Panels;
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PTM Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. [UniProt]
Title Download Link
ARG82622 Human IL16 ELISA Kit User manual download icon Download