ARG80162

Human IL17 / IL17A (High sensitive) ELISA kit

Human IL17 / IL17A (High sensitive) ELISA kit for ELISA and Human

Immune System kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80162-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80162-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80162-003 Standard diluent buffer 20 ml 4°C
ARG80162-004 Antibody conjugate concentrate 400 µl 4°C
ARG80162-005 Antibody diluent buffer 16 ml 4°C
ARG80162-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80162-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80162-008 20X Wash buffer 50 ml 4°C
ARG80162-009 TMB substrate 12ml 4°C (Protect from light)
ARG80162-010 STOP solution 12ml 4°C
ARG80162-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80162 Human IL17 / IL17A (High sensitive) ELISA Kit is a high sensitive Enzyme Immunoassay kit for the quantification of Human IL17 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IFN- gamma, IL-17B, IL-10, IL-17C, IL-12, IL-17D, IL-16, IL-17F;mouse IL-17
Target Name IL17 / IL17A
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 3.13 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 6.25 - 400 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names IL-17A; Interleukin-17A; IL-17; CTLA8; IL17; CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3605 Human IL17A

Swiss-port # Q16552 Human Interleukin-17A

Gene Symbol IL17A
Gene Full Name interleukin 17A
Background The originally described IL-17 protein, now known as IL-17A, is a disulfide linked homodimer, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells. Recombinant murine IL-17A is a 30.0 kDa disulfide-linked homodimer of two 133 amino acid polypeptide chains.
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Research Area Immune System kit
PTM Found both in glycosylated and nonglycosylated forms.

Specific References

IL-17A promotes the formation of deep vein thrombosis in a mouse model.

ELISA / Human

Ding Peiwu et al.
Int Immunopharmacol.,  (2018)

publication_link

 

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Title Download Link
ARG80162 Human IL17 / IL17A (High sensitive) ELISA kit User's manual download icon Download