ARG70033
Human IL17 / IL17A recombinant protein (Active) (His-tagged, C-ter)
Human IL17 / IL17A recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL17 / IL17A recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL17 / IL17A recombinant protein |
| Species | Human |
| A.A. Sequence | MIVKA GITIP RNPGC PNSED KNFPR TVMVN LNIHN RNTNT NPKRS SDYYN RSTSP WNLHR NEDPE RYPSV IWEAK CRHLG CINAD GNVDY HMNSV PIQQE ILVLR REPPH CPNSF RLEKI LVSVG CTCVT PIVHH VA with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-6 secretion in 3T3 cells. The ED50 for this effect is <6 ng/mL. |
| Alternate Names | IL-17A; Interleukin-17A; IL-17; CTLA8; IL17; CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL17A |
|---|---|
| Gene Full Name | interleukin 17A |
| Background | The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008] |
| Function | Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL17 antibodies; IL17 ELISA Kits; IL17 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 |
| PTM | Found both in glycosylated and nonglycosylated forms. [UniProt] |
