ARG70060
Human IL17B recombinant protein (Active) (His-tagged, C-ter)
Human IL17B recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL17B recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL17B |
| Species | Human |
| A.A. Sequence | MQPRS PKSKR KGQGR PGPLA PGPHQ VPLDL VSRMK PYARM EEYER NIEEM VAQLR NSSEL AQRKC EVNLQ LWMSN KRSLS PWGYS INHDP SRIPV DLPEA RCLCL GCVNP FTMQE DRSMV SVPVF SQVPV RRRLC PPPPR TGPCR QRAVM ETIAV GCTCI F with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 49 ng/ml. |
| Alternate Names | IL-17B; Interleukin-20; Interleukin-17B; IL-20; Neuronal interleukin-17-related factor; NIRF; Cytokine Zcyto7; ZCYTO7 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL17B |
|---|---|
| Gene Full Name | interleukin 17B |
| Background | The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. [provided by RefSeq, Jul 2008] |
| Function | Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
