ARG70061
Human IL17F recombinant protein (Active) (His-tagged, C-ter)
Human IL17F recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL17F recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL17F |
| Species | Human |
| A.A. Sequence | MRKIP KVGHT FFQKP ESCPP VPGGS MKLDI GIINE NQRVS MSRNI ESRST SPWNY TVTWD PNRYP SEVVQ AQCRN LGCIN AQGKE DISMN SVPIQ QETLV VRRKH QGCSV SFQLE KVLVT VGCTC VTPVI HHVQ with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-6 secretion in 3T3 cells. The ED50 for this effect is < 20 ng/ml. |
| Alternate Names | Interleukin-17F; IL-17F; CANDF6; ML-1; ML1; Cytokine ML-1 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | 20 mM Sodium acetate (pH 4.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL17F |
|---|---|
| Gene Full Name | interleukin 17F |
| Background | The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008] |
| Function | Stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
