ARG70290

Human IL17RA recombinant protein (Active) (ECD) (His-tagged, C-ter)

Human IL17RA recombinant protein (Active) (ECD) (His-tagged, C-ter) for SDS-PAGE,Functional study,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Active Human IL17RA recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, FuncSt, SDS-PAGE
Target Name IL17RA (ECD)
Species Human
A.A. Sequence Leu33 - Trp320 of Human IL17RA (NP_055154.3) with 6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured by its ability to inhibit IL-17A induced IL-6 secretion by HeLa cells. The ED50 for this effect is 0.02-0.1 µg/ml in the presence of 10 ng/ml recombinant human IL-17A.
Alternate Names Interleukin-17 receptor A; IL-17RA; IL-17 receptor A; hIL-17R; CANDF5; CDw217; IL17R; CD217; CD antigen CD217

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant human IL17RA , His Tag at 2 µg/ml (100 µl/well) can bind Recombinant human IL-17A, His Tag with a linear range of 31.25-65 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23765 Human IL17RA

Swiss-port # Q96F46 Human Interleukin-17 receptor A

Gene Symbol IL17RA
Gene Full Name interleukin 17 receptor A
Background Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2014]
Function Receptor for IL17A (PubMed:17911633, PubMed:9367539). Receptor for IL17F (PubMed:19838198, PubMed:17911633). Binds to IL17A with higher affinity than to IL17F (PubMed:17911633). Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC (PubMed:16785495). Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC (PubMed:18684971). Receptor for IL17C as part of a heterodimeric complex with IL17RE (PubMed:21993848). Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6 (PubMed:16785495, PubMed:17911633, PubMed:18684971). [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt]
Calculated MW 96 kDa
PTM Glycosylated. [UniProt]