ARG80138

Human IL18 ELISA Kit

Human IL18 ELISA Kit for ELISA and Human

Cancer kit; Immune System kit; Metabolism kit
publication_link Publication2

Component

Cat No Component Name Package Temp
ARG80138-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80138-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80138-003 Standard diluent buffer 20 ml 4°C
ARG80138-004 Antibody conjugate concentrate 400 µl 4°C
ARG80138-005 Antibody diluent buffer 16 ml 4°C
ARG80138-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80138-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80138-008 20X Wash buffer 50 ml 4°C
ARG80138-009 TMB substrate 12ml 4°C (Protect from light)
ARG80138-010 STOP solution 12ml 4°C
ARG80138-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80138 Human IL18 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL18 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IFN- alpha, IFN- gamma, IL-1 beta, IL-4, IL-5, IL-6, IL-l0, IL-12, GM-CSF ;mouse IL-18
Target Name IL18
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL18
Gene Full Name interleukin 18
Background The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt]
Highlight Related products:
IL18 antibodies; IL18 ELISA Kits;
Related news:
Activation of IL-1 beta and IL-18 by Canonical Inflammasome Pathway
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Cancer kit; Immune System kit; Metabolism kit
PTM The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form.

Specific References

Peroxiredoxin 1 regulates crosstalk between pyroptosis and autophagy in oral squamous cell carcinoma leading to a potential pro-survival

ELISA / Human / Culture supernatant

Meilin Ye et al.
Cell Death Discov.,  (2023)

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Role of Different Salmonella enterica Serotypes in THP-1 Macrophage-Derived Foam Cell Autophagy and Inflammatory Response.

ELISA / Human

Wong Min-Yi et al.
Sains Malaysiana.,  (2019)

publication_link

 

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Title Download Link
ARG80138 Human IL18 ELISA Kit User manual download icon Download