ARG81544

Human IL18 binding protein ELISA Kit

Human IL18 binding protein ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81544-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81544-002 Standard 2 X 10 ng/vial 4°C
ARG81544-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81544-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81544-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81544-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81544-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81544-008 25X Wash buffer 20 ml 4°C
ARG81544-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81544-010 STOP solution 10 ml (Ready to use) 4°C
ARG81544-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81544 Human IL18 binding protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL18 binding protein in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name IL18 binding protein
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 46.85 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 93.7 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 6.7%
Alternate Names IL18BPa; Tadekinig-alfa; IL-18BP; Interleukin-18-binding protein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10068 Human IL18BP

Swiss-port # O95998 Human Interleukin-18-binding protein

Gene Symbol IL18BP
Gene Full Name interleukin 18 binding protein
Background The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
Function Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. [UniProt]
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IL18 binding protein antibodies; IL18 binding protein ELISA Kits;
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PTM N- and O-glycosylated. O-glycosylated with core 1-like and core 2-like glycans. O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor). N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); N-glycan at Asn-147: dHex1Hex5HexNAc4. [UniProt]
Title Download Link
ARG81544 Human IL18 binding protein ELISA Kit User's manual download icon Download