ARG70062
Human IL18 recombinant protein (Active) (His-tagged, C-ter)
Human IL18 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL18 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL18 |
| Species | Human |
| A.A. Sequence | MYFGK LESKL SVIRN LNDQV LFIDQ GNRPL FEDMT DSDCR DNAPR TIFII SMYKD SQPRG MAVTI SVKCE KISTL SCENK IISFK EMNPP DNIKD TKSDI IFFQR SVPGH DNKMQ FESSS YEGYF LACEK ERDLF KLILK KEDEL GDRSI MFTVQ NED with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is < 5 ng/ml. |
| Alternate Names | Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL18 |
|---|---|
| Gene Full Name | interleukin 18 |
| Background | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| Function | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
