ARG70063
Human IL19 recombinant protein (Active) (His-tagged, C-ter)
Human IL19 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL19 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL19 |
| Species | Human |
| A.A. Sequence | MLRRC LISTD MHHIE ESFQE IKRAI QAKDT FPNVT ILSTL ETLQI IKPLD VCCVT KNLLA FYVDR VFKDH QEPNP KILRK ISSIA NSFLY MQKTL RQCQE QRQCH CRQEA TNATR VIHDN YDQLE VHAAA IKSLG ELDVF LAWIN KNHEV MSSA with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is < 1.2 ng/ml. |
| Alternate Names | Interleukin-19; IL-10C; ZMDA1; IL-19; MDA1; NG.1; Melanoma differentiation-associated protein-like protein |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL19 |
|---|---|
| Gene Full Name | interleukin 19 |
| Background | The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Function | May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis (By similarity). [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
