ARG70044
Human IL1RA recombinant protein (Active) (His-tagged, C-ter)
Human IL1RA recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL1RA recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL1RA |
| Species | Human |
| A.A. Sequence | MRPSG RKSSK MQAFR IWDVN QKTFY LRNNQ LVAGY LQGPN VNLEE KIDVV PIEPH ALFLG IHGGK MCLSC VKSGD ETRLQ LEAVN ITDLS ENRKQ DKRFA FIRSD SGPTT SFESA ACPGW FLCTA MEADQ PVSLT NMPDE GVMVT KFYFQ EDE with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 cells. The ED50 for this effect is < 50 ng/ml. |
| Alternate Names | IL1 inhibitor; Interleukin-1 receptor antagonist protein; ICIL-1RA; MVCD4; IL1RA; DIRA; IL-1RN; Anakinra; IL1F3; IRAP; IL-1ra; IL-1ra3 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL1RN |
|---|---|
| Gene Full Name | interleukin 1 receptor antagonist |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. [UniProt] |
| Cellular Localization | Isoform 1: Secreted. Isoform 2: Cytoplasm. Isoform 3: Cytoplasm. Isoform 4: Cytoplasm. [UniProt] |
