ARG70064
Human IL20 recombinant protein (Active) (His-tagged, C-ter)
Human IL20 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL20 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL20 |
| Species | Human |
| A.A. Sequence | MLKTL NLGSC VIATN LQEIR NGFSE IRGSV QAKDG NIDIR ILRRT ESLQD TKPAN RCCLL RHLLR LYLDR VFKNY QTPDH YTLRK ISSLA NSFLT IKKDL RLCHA HMTCH CGEEA MKKYS QILSH FEKLE PQAAV VKALG ELDIL LQWME ETE with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human IL-20R alpha and IL-20R beta. The ED50 for this effect is < 0.2 ng/ml. |
| Alternate Names | Interleukin-20; IL-20; IL10D; Cytokine Zcyto10; ZCYTO10 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL20 |
|---|---|
| Gene Full Name | interleukin 20 |
| Background | The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008] |
| Function | Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
