ARG70067
Human IL23A / IL23 p19 recombinant protein (Active) (His-tagged, N-ter)
Human IL23A / IL23 p19 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human IL23A / IL23 p19 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL23A / IL23 p19 |
| Species | Human |
| A.A. Sequence | RAVPG GSSPA WTQCQ QLSQK LCTLA WSAHP LVGHM DLREE GDEET TNDVP HIQCG DGCDP QGLRD NSQFC LQRIH QGLIF YEKLL GSDIF TGEPS LLPDS PVGQL HASLL GLSQL LQPEG HHWET QQIPS LSPSQ PWQRL LLRFK ILRSL QAFVA VAARV FAHGA ATLSP with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured by its ability to induce IL-17 secretion in mouse splenocytes. The ED50 for this effect is < 0.5 ng/ml |
| Alternate Names | IL-23A; IL-23; IL-23 subunit alpha; IL23P19; IL-23p19; Interleukin-23 subunit alpha; IL-23-A; P19; SGRF; Interleukin-23 subunit p19 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL23A |
|---|---|
| Gene Full Name | interleukin 23, alpha subunit p19 |
| Background | This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008] |
| Function | Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. [UniProt] |
| Cellular Localization | Secreted. Note=Secreted upon association with IL12B. [UniProt] |
