ARG70068
Human IL24 recombinant protein (Active) (His-tagged, C-ter)
Human IL24 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL24 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL24 |
| Species | Human |
| A.A. Sequence | MQEFH FGPCQ VKGVV PQKLW EAFWA VKDTM QAQDN ITSAR LLQQE VLQNV SDAES CYLVH TLLEF YLKTV FKNYH NRTVE VRTLK SFSTL ANNFV LIVSQ LQPSQ EN AE SCYLV HTLLE FYLKT VFKNY HNRTV EVRTL KSFST LANNF VLIVS QLQPS QEN with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human IL-20R alpha and IL-20R beta. The ED50 for this effect is < 100 pg/ml. |
| Alternate Names | IL10B; Interleukin-24; Melanoma differentiation-associated gene 7 protein; MDA7; MDA-7; C49A; ST16; FISP; Suppression of tumorigenicity 16 protein; IL-24; MOB5 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL24 |
|---|---|
| Gene Full Name | interleukin 24 |
| Background | This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | Ubiquitination at Lys-122 promotes proteasomal degradation. [UniProt] |
