ARG80165

Human IL25 / IL17E ELISA Kit

Human IL25 / IL17E ELISA Kit for ELISA and Human

Immune System kit

Component

Cat No Component Name Package Temp
ARG80165-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80165-002 Standard (Lyophilized) 3 X 2 ng/vial 4°C
ARG80165-003 Standard diluent buffer 20 ml 4°C
ARG80165-004 Antibody conjugate concentrate 400 µl 4°C
ARG80165-005 Antibody diluent buffer 16 ml 4°C
ARG80165-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80165-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80165-008 20X Wash buffer 50 ml 4°C
ARG80165-009 TMB substrate 12ml 4°C (Protect from light)
ARG80165-010 STOP solution 12ml 4°C
ARG80165-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80165 Human IL25 / IL17E ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL25 / IL17E in serum, plasma and cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IFN- gamma, IL-8(72a.a), IL-8(77a.a), IL-10, IL-12, IL-12p40, IL-17A, IL-17D, IL- 17F;mouse IL-17A, IL-17F
Target Name IL25 / IL17E
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 15 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names IL17E; Interleukin-17E; IL-25; Interleukin-25; IL-17E

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64806 Human IL25

Swiss-port # Q9H293 Human Interleukin-25

Gene Symbol IL25
Gene Full Name interleukin 25
Background The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17. This cytokine can induce NF-kappaB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17BR. Studies of the similar gene in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
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Research Area Immune System kit
Title Download Link
ARG80165 Human IL25 / IL17E ELISA Kit User's manual download icon Download