ARG70069

Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter)

Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human IL25 / IL17E recombinant protein
Tested Application SDS-PAGE
Target Name IL25 / IL17E
Species Human
A.A. Sequence MYSHW PSCCP SKGQD TSEEL LRWST VPVPP LEPAR PNRHP ESCRA SEDGP LNSRA ISPWR YELDR DLNRL PQDLY HARCL CPHCV SLQTG SHMDP RGNSE LLYHN QTVFY RRPCH GEKGT HKGYC LERRL YRVSL ACVCV RPRVM G with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 5 ng/ml. Determined by its ability to induce CXCL1 secretion in HT29 cells. The ED50 for this effect is < 1 ng/ml.
Alternate Names IL17E; Interleukin-17E; IL-25; Interleukin-25; IL-17E

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL25
Gene Full Name interleukin 25
Background The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Function Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. [UniProt]
Cellular Localization Secreted. [UniProt]