ARG70069
Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter)
Human IL25 / IL17E recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL25 / IL17E recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL25 / IL17E |
| Species | Human |
| A.A. Sequence | MYSHW PSCCP SKGQD TSEEL LRWST VPVPP LEPAR PNRHP ESCRA SEDGP LNSRA ISPWR YELDR DLNRL PQDLY HARCL CPHCV SLQTG SHMDP RGNSE LLYHN QTVFY RRPCH GEKGT HKGYC LERRL YRVSL ACVCV RPRVM G with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 5 ng/ml. Determined by its ability to induce CXCL1 secretion in HT29 cells. The ED50 for this effect is < 1 ng/ml. |
| Alternate Names | IL17E; Interleukin-17E; IL-25; Interleukin-25; IL-17E |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL25 |
|---|---|
| Gene Full Name | interleukin 25 |
| Background | The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Function | Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
