ARG70070

Human IL26 recombinant protein (Active) (His-tagged, C-ter)

Human IL26 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human IL26 recombinant protein
Tested Application SDS-PAGE
Target Name IL26
Species Human
A.A. Sequence MKHKQ SSFTK SCYPR GTLSQ AVDAL YIKAA WHKAT IPEDR IKNIR LLKKK TKKQF MKNCQ FQEQL LSFFM EDVFG QLQLQ GCKKI RFVED FHSLR QKLSH CISCA SSARE MKSIT RMKRI FYRIG NKGIY KAISE LDILL SWIKK LLESS Q with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce IL-10 secretion in COLO205 cells. The ED50 for this effect is < 150 ng/ml.
Alternate Names AK155; Protein AK155; IL-26; Interleukin-26

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL26
Gene Full Name interleukin 26
Background This gene was identified by its overexpression specifically in herpesvirus samimiri-transformed T cells. The encoded protein is a member of the IL10 family of cytokines. It is a secreted protein and may function as a homodimer. This protein is thought to contribute to the transformed phenotype of T cells after infection by herpesvirus samimiri. [provided by RefSeq, Jul 2008]
Function May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin. [UniProt]
Cellular Localization Secreted. [UniProt]