ARG70074
Human IL29 / IFN lambda recombinant protein (Active) (His-tagged, C-ter)
Human IL29 / IFN lambda recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL29 / IFN lambda recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL29 / IFN lambda |
| Species | Human |
| A.A. Sequence | MPTSK PTTTG KGCHI GRFKS LSPQE LASFK KARDA LEESL KLKNW SCSSP VFPGN WDLRL LQVRE RPVAL EAELA LTLKV LEAAA GPALE DVLDQ PLHTL HHILS QLQAC IQPQP TAGPR PRGRL HHWLH RLQEA PKKES AGCLE ASVTF NLFRL LTRDL KYVAD GNLCL RTSTH PEST with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in HuH7 cells. The ED50 for this effect is < 6 ng/ml. |
| Alternate Names | IFN-lambda-1; IL-29; Interferon lambda-1; Interleukin-29; Cytokine Zcyto21; IL29 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNL1 |
|---|---|
| Gene Full Name | interferon, lambda 1 |
| Background | This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008] |
| Function | Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL29 antibodies; IL29 ELISA Kits; IL29 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 |
