ARG80104

Human IL2sR alpha ELISA Kit

Human IL2sR alpha ELISA Kit for ELISA and Human

Immune System kit

Component

Cat No Component Name Package Temp
ARG80104-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80104-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80104-003 Standard diluent buffer 20 ml 4°C
ARG80104-004 Antibody conjugate concentrate 400 µl 4°C
ARG80104-005 Antibody diluent buffer 16 ml 4°C
ARG80104-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80104-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80104-008 20X Wash buffer 50 ml 4°C
ARG80104-009 TMB substrate 12ml 4°C (Protect from light)
ARG80104-010 STOP solution 12ml 4°C
ARG80104-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80104 Human IL2sR alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL2sR alpha in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with other soluble cytokine
Target Name CD25
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 16 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Highlight Related products:
CD25 antibodies; CD25 ELISA Kits; CD25 recombinant proteins;
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Simplify the ELISA analysis by GainData
Research Area Immune System kit
Title Download Link
ARG80104 Human IL2sR alpha ELISA Kit User's manual download icon Download