ARG70075
Human IL31 recombinant protein (Active) (His-tagged, C-ter)
Human IL31 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL31 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL31 |
| Species | Human |
| A.A. Sequence | MSHTL PVRLL RPSDD VQKIV EELQS LSKML LKDVE EEKGV LVSQN YTLPC LSPDA QPPNN IHSPA IRAYL KTIRQ LDNKS VIDEI IEHLD KLIFQ DAPET NISVP TDTHE CKRFI LTISQ QFSEC MDLAL KSLTS GAQQA TT with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Measured by its ability to induce STAT3 activation in U-87 cells. The ED50 for this effect is < 20 ng/ml. |
| Alternate Names | IL-31; Interleukin-31 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL31 |
|---|---|
| Gene Full Name | interleukin 31 |
| Background | IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM, Mar 2008] |
| Function | Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
