ARG70076
Human IL32 (isoform alpha) recombinant protein (Active) (His-tagged, C-ter)
Human IL32 (isoform alpha) recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL32 (isoform alpha) recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL32 (isoform alpha) |
| Species | Human |
| A.A. Sequence | MCFPK VLSDD MKKLK ARMHQ AIERF YDKMQ NAESG RGQVM SSLAE LEDDF KEGYL ETVAA YYEEQ HPELT PLLEK ERDGL RCRGN RSPVP DVEDP ATEEP GESFC DKSYG APRGD KEELT PQKCS EPQSS K with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce TNF alpha secretion in RAW264.7 cells. The ED50 for this effect is < 10 μg/ml. |
| Alternate Names | IL-32gamma; IL-32beta; IL-32delta; Interleukin-32; TAIFa; TAIFc; TAIFb; NK4; TAIFd; IL-32; TAIF; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor; IL-32alpha |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL32 |
|---|---|
| Gene Full Name | interleukin 32 |
| Background | This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
