ARG82953
Human IL36 beta ELISA kit
Human IL36 beta ELISA kit for ELISA and Human
Overview
| Product Description | ARG82953 Human IL36 beta ELISA kit is an Enzyme Immunoassay kit for the quantification of Human IL36 beta in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | IL36 beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 μl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | FIL1; IL1-ETA; FILI-(ETA); IL-1F8; Interleukin-1 homolog 2; IL1H2; IL-1H2; FIL1-(ETA); Interleukin-1 eta; IL-1 eta; IL1F8; FIL1 eta; Interleukin-36 beta; Interleukin-1 family member 8; FIL1H |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | 4°C |
| Note | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
Bioinformation
| Gene Symbol | IL36B |
|---|---|
| Gene Full Name | interleukin 36, beta |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL36 beta antibodies; IL36 beta ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). [UniProt] |
| Title | Download Link |
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| ARG82953 Human IL36 beta ELISA kit User manual |
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