ARG70080
Human IL36 beta recombinant protein (Active) (His-tagged, C-ter)
Human IL36 beta recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL36 beta recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL36 beta |
| Species | Human |
| A.A. Sequence | MREAA PKSYA IRDSR QMVWV LSGNS LIAAP LSRSI KPVTL HLIAC RDTEF SDKEK GNMVY LGIKG KDLCL FCAEI QGKPT LQLKE KNIMD LYVEK KAQKP FLFFH NKEGS TSVFQ SVSYP GWFIA TSTTS GQPIF LTKER GITNN TNFYL DSVE with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.2 ng/ml. |
| Alternate Names | FIL1; IL1-ETA; FILI-(ETA); IL-1F8; Interleukin-1 homolog 2; IL1H2; IL-1H2; FIL1-(ETA); Interleukin-1 eta; IL-1 eta; IL1F8; FIL1 eta; Interleukin-36 beta; Interleukin-1 family member 8; FIL1H |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL36B |
|---|---|
| Gene Full Name | interleukin 36, beta |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). [UniProt] |
