ARG82785

Human IL36 gamma ELISA Kit

Human IL36 gamma ELISA Kit for ELISA and Human

Overview

Product Description ARG82785 Human IL36 gamma ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL36 gamma in serum, plasma (EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No cross-reactivity with Human IL1 RAcP/Fc Chimera, IL1 Rrp2/Fc Chimera, IL36 alpha (aa. 6-158), IL36 beta (aa. 5-157), IL36RA and Mouse IL36 gamma.
Target Name IL36 gamma
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 18.75 pg/ml
Sample Type Serum, plasma (EDTA) and cell culture supernatants.
Standard Range 37.5 - 2400 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: < 10%
Inter-Assay CV: < 10%
Alternate Names IL-1 epsilon; IL1H1; Interleukin-1 homolog 1; IL-1-related protein 2; Interleukin-36 gamma; IL-1H1; IL-1F9; IL1F9; IL-1RP2; IL1RP2; IL1E; Interleukin-1 family member 9; Interleukin-1 epsilon

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56300 Human IL36G

Swiss-port # Q9NZH8 Human Interleukin-36 gamma

Gene Symbol IL36G
Gene Full Name interleukin 36, gamma
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, May 2019]
Function Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. [UniProt]
Cellular Localization Secreted. [UniProt]
PTM N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). [UniProt]
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