ARG70082
Human IL36RN recombinant protein (Active) (His-tagged, C-ter)
Human IL36RN recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL36RN recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL36RN |
| Species | Human |
| A.A. Sequence | MVLSG ALCFR MKDSA LKVLY LHNNQ LLAGG LHAGK VIKGE EISVV PNRWL DASLS PVILG VQGGS QCLSC GVGQE PTLTL EPVNI MELYL GAKES KSFTF YRRDM GLTSS FESAA YPGWF LCTVP EADQP VRLTQ LPENG GWNAP ITDFY FQQCD with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells. The ED50 for this effect is < 2 ng/ml in the presence of 500 ng/ml of recombinant human IL-36 gamma. |
| Alternate Names | IL1L1; FIL1 delta; IL-1 delta; IL-1F5; IL-1L1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; IL-1-related protein 3; PSORS14; IL36RA; Interleukin-1 HY1; Interleukin-1 family member 5; IL-1RP3; IL-1HY1; IL1HY1; IL1F5; IL1RP3; FIL1; IL-36Ra; FIL1D; IL-1ra homolog 1; PSORP; FIL1(DELTA); Interleukin-36 receptor antagonist protein; Interleukin-1 delta |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL36RN |
|---|---|
| Gene Full Name | interleukin 36 receptor antagonist |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
| Function | Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
