ARG70083
Human IL37 / IL1F7 / FIL1 zeta recombinant protein (Active) (His-tagged, C-ter)
Human IL37 / IL1F7 / FIL1 zeta recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL37 / IL1F7 / FIL1 zeta recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL37 / IL1F7 / FIL1 zeta |
| Species | Human |
| A.A. Sequence | MKNLN PKKFS IHDQD HKVLV LDSGN LIAVP DKNYI RPEIF FALAS SLSSA SAEKG SPILL GVSKG EFCLY CDKDK GQSHP SLQLK KEKLM KLAAQ KESAR RPFIF YRAQV GSWNM LESAA HPGWF ICTSC NCNEP VGVTD KFENR KHIEF SFQPV CKAEM SPSEV SD with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is < 0.9 μg/ml. |
| Alternate Names | Interleukin-1 zeta; IL-1 zeta; FIL1; Interleukin-37; IL-1X; IL-1F7; FIL1 zeta; IL-1H4; Interleukin-1 homolog 4; FIL1Z; IL1H4; Interleukin-1 family member 7; Interleukin-23; IL-37; FIL1(ZETA); IL-1H; IL-1RP1; IL1RP1; IL1F7; Interleukin-1-related protein |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL37 |
|---|---|
| Gene Full Name | interleukin 37 |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). This cytokine also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18. This gene along with eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Nucleus. Secreted. Note=Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions (PubMed:20935647). Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation (PubMed:18390730). [UniProt] |
| PTM | Proteolytically converted to the mature form by CASP1. [UniProt] |
