ARG70048

Human IL5 recombinant protein (Active) (His-tagged, C-ter)

Human IL5 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human IL5 recombinant protein
Tested Application SDS-PAGE
Target Name IL5
Species Human
A.A. Sequence MIPTE IPTSA LVKET LALLS THRTL LIANE TLRIP VPVHK NHQLC TEEIF QGIGT LESQT VQGGT VERLF KNLSL IKKYI DGQKK KCGEE RRRVN QFLDY LQEFL GVMNT EWIIE S with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.3 ng/ml.
Alternate Names Eosinophil differentiation factor; EDF; IL-5; TRF; T-cell replacing factor; B-cell differentiation factor I; Interleukin-5

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL5
Gene Full Name interleukin 5
Background This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
Function Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. [UniProt]
Cellular Localization Secreted. [UniProt]