ARG70288
Human IL6R alpha recombinant protein (ECD) (His-tagged, C-ter)
Human IL6R alpha recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human IL6R alpha recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | IL6R alpha (ECD) |
| Species | Human |
| A.A. Sequence | Leu20 - Asp358 of Human IL6R alpha (NP_000556.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | IL-6R-alpha; CD126; IL6RA; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL6Q; CD antigen CD126; IL-6R 1; IL-6RA; gp80; IL6RQ; Interleukin-6 receptor subunit alpha; IL-6R-1; Membrane glycoprotein 80 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human IL6R at 1 ug/ml (100 µl/well) can bind Human IL-6 with a linear range of 2-15ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 92% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08887 Human Interleukin-6 receptor subunit alpha |
|---|---|
| Gene Symbol | IL6R |
| Gene Full Name | interleukin 6 receptor |
| Background | This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9. [provided by RefSeq, May 2011] |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal (PubMed:28265003). Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. [UniProt] |
| Cellular Localization | Isoform 1: Basolateral cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
| Calculated MW | 52 kDa |
| PTM | A short soluble form may also be released from the membrane by proteolysis. [UniProt] |
