ARG70052
Human IL9 recombinant protein (Active) (His-tagged, N-ter)
Human IL9 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human IL9 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL9 |
| Species | Human |
| A.A. Sequence | QGCPT LAGIL DINFL INKMQ EDPAS KCHCS ANVTS CLCLG IPSDN CTRPC FSERL SQMTN TTMQT RYPLI FSRVK KSVEV LKNNK CPYFS CEQPC NQTTA GNALT FLKSL LEIFQ KEKMR GMRGK I with polyhistidine tag at the N-terminus. |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce proliferation in MO7e cells. The ED50 for this effect is < 0.25 ng/ml. The specific activity of recombinant human IL-9 is approximately > 5 x 10^6 IU/mg. |
| Alternate Names | IL-9; HP40; Interleukin-9; T-cell growth factor P40; Cytokine P40; P40 |
Properties
| Form | Powder |
|---|---|
| Purification | Ni-NTA chromatography. |
| Purification Note | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL9 |
|---|---|
| Gene Full Name | interleukin 9 |
| Background | The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008] |
| Function | Supports IL-2 independent and IL-4 independent growth of helper T-cells. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
