ARG70052

Human IL9 recombinant protein (Active) (His-tagged, N-ter)

Human IL9 recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (N-ter) Active Human IL9 recombinant protein
Tested Application SDS-PAGE
Target Name IL9
Species Human
A.A. Sequence QGCPT LAGIL DINFL INKMQ EDPAS KCHCS ANVTS CLCLG IPSDN CTRPC FSERL SQMTN TTMQT RYPLI FSRVK KSVEV LKNNK CPYFS CEQPC NQTTA GNALT FLKSL LEIFQ KEKMR GMRGK I with polyhistidine tag at the N-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce proliferation in MO7e cells. The ED50 for this effect is < 0.25 ng/ml. The specific activity of recombinant human IL-9 is approximately > 5 x 10^6 IU/mg.
Alternate Names IL-9; HP40; Interleukin-9; T-cell growth factor P40; Cytokine P40; P40

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 8.0)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL9
Gene Full Name interleukin 9
Background The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]
Function Supports IL-2 independent and IL-4 independent growth of helper T-cells. [UniProt]
Cellular Localization Secreted. [UniProt]