ARG80175

Human IgG4 ELISA Kit

Human IgG4 ELISA Kit for ELISA and Human

Immune System kit

Component

Cat No Component Name Package Temp
ARG80175-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80175-002 Standard (Lyophilized) 3 X 1.5 μg/vial 4°C
ARG80175-003 Standard diluent buffer 40 ml 4°C
ARG80175-004 Antibody conjugate concentrate 400 µl 4°C
ARG80175-005 Antibody diluent buffer 16 ml 4°C
ARG80175-008 20X Wash buffer 50 ml 4°C
ARG80175-009 TMB substrate 12ml 4°C (Protect from light)
ARG80175-010 STOP solution 12ml 4°C
ARG80175-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80175 Human IgG4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IgG4 in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IgG1, IgG2, IgG3
Target Name IgG4
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 ng/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 15.6 - 1000 ng/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names Immunoglobulin G4

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background IgG4 is present in very low levels in children younger than 10 years of age, so IgG4 deficiencies are not usually diagnosed before age 10. IgG4 may be undetectable in the serum of many “normal” adult individuals, and therefore low IgG4 alone is insufficient evidence of an antibody deficiency disorder requiring Ig replacement. IgG4–related systemic disease (IgG4-RSD), also known as hyper-IgG4 disease and IgG4-related disease is a disease in which inflammatory cells cause fibrosis, the deposition of connective tissue, in one or more organs. The disease is so named because the antibody subtype IgG4 can be detected on tissue samples and often at elevated levels in the bloodstream. The association with IgG4 is a relatively recent finding, and the condition has been described under numerous other names in the past. Diseases such asautoimmune pancreatitis, retroperitoneal fibrosis, mediastinal fibrosis, Riedel's thyroiditis, Mikulicz's syndrome, Kuttner's tumor and inflammatory pseudotumor are now regarded as forms of IgG4-RSD.
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Research Area Immune System kit
Title Download Link
ARG80175 Human IgG4 ELISA Kit User's manual download icon Download