ARG80929
Human Inflammatory Cytokine Multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha)
Human Inflammatory Cytokine Multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha) for ELISA and Human
Immune System kit; Metabolism kit
Publication2
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80929-001 | Antibody-coated microplate | 8 X 12 strips coated with 8 different antibodies. | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80929-002 | Standard mixtures | 2 vials (Lyophilized) | 4°C |
| ARG80929-003 | Biotin conjugate mixture | 6 ml (Ready-to-use) | 4°C |
| ARG80929-004 | HRP conjugate mixture | 11 ml (Ready-to-use) | 4°C |
| ARG80929-005 | Standard Diluent I (for serum/plasma testing) | 25 ml (Ready-to-use) | 4°C |
| ARG80929-006 | Standard Diluent II (for cell culture supernatant testing) | 25 ml (Ready-to-use) | 4°C |
| ARG80929-007 | 20X Wash buffer | 60 ml | 4°C |
| ARG80929-008 | Substrate A | 10 ml | 4°C (Protect from light) |
| ARG80929-009 | Substrate B | 10 ml | 4°C (Protect from light) |
| ARG80929-010 | STOP solution | 14 ml (Ready-to-use) | 4°C |
Overview
| Product Description | ARG80929 Human Inflammatory Cytokine Multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha) is an Enzyme Immunoassay kit for the semi-quantification of IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha in serum, plasma, cell culture supernatant and other biological samples. See all Multiplex ELISA kits |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Inflammatory Cytokine Multiplex |
| Conjugation | HRP |
| Conjugation Note | At a wavelength of 450 nm |
| Detection Range | IL1 alpha: 20.32 - 650 pg/ml IL1 beta: 23.44 - 750 pg/ml IL6: 7.82 - 250 pg/ml IL8: 25 - 800 pg/ml GM-CSF: 17.19 - 550 pg/ml IFN-gamma: 10.94 - 350 pg/ml MCAF: 25 - 800 pg/ml TNF-alpha: 17.19 - 550 pg/ml |
| Sample Type | Serum, plasma, cell culture supernatant and other biological samples. |
| Sample Volume | 100 µl |
Application Instructions
| Assay Time | 3.5 hour |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Inflammation is body's protective response to foreign subjects, pathogens, tissue damage, autoimmune and other harmful stimuli. It is initiated by production of a cascade of chemicals and cytokines in affected area. These pro-inflammatory mediators result in vasodilation, increased vascular permeability, influx of blood, plasma leakage, neutrophil and macrophage infiltration and activation. Inflammatory reaction plays an important role in limiting foreign substance and engulfing pathogens and tissue debris. Acute inflammation is the initial phase to eliminate invaded foreign substances, pathogens and other harmful stimuli. If the stimuli persist, chronic inflammation will evolve. Progressive tissue destruction and shifting cell types occurs simultaneously during chronic inflammation. The cytokine profiles that regulate the procedure are different depending on the causes, location and progress of the inflammation. |
|---|---|
| Highlight | Related products: Inflammation antibodies; Inflammation Duos / Panels; Related news: HMGB1 in inflammation Inflammatory Cytokines Exploring Antiviral Immune Response New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit; Metabolism kit |
Specific References
Long-Term Changes in Cytokine and Ghrelin Levels Following Laparoscopic Sleeve Gastrectomy in Obese Individuals
ELISA / Human / Tissue lysate
Antimicrobial and Immunomodulatory Activity of Herb Extracts Used in Burn Wound Healing: "San Huang Powder"
ELISA / Human / Cell culture supernatants
| Title | Download Link |
|---|---|
| ARG80929 Human Inflammatory Cytokine multiplex ELISA Kit User manual |
 Download
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