ARG80929

Human Inflammatory Cytokine Multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha)

Human Inflammatory Cytokine Multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha) for ELISA and Human

Immune System kit; Metabolism kit
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Component

Cat No Component Name Package Temp
ARG80929-001 Antibody-coated microplate 8 X 12 strips coated with 8 different antibodies. 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80929-002 Standard mixtures 2 vials (Lyophilized) 4°C
ARG80929-003 Biotin conjugate mixture 6 ml (Ready-to-use) 4°C
ARG80929-004 HRP conjugate mixture 11 ml (Ready-to-use) 4°C
ARG80929-005 Standard Diluent I (for serum/plasma testing) 25 ml (Ready-to-use) 4°C
ARG80929-006 Standard Diluent II (for cell culture supernatant testing) 25 ml (Ready-to-use) 4°C
ARG80929-007 20X Wash buffer 60 ml 4°C
ARG80929-008 Substrate A 10 ml 4°C (Protect from light)
ARG80929-009 Substrate B 10 ml 4°C (Protect from light)
ARG80929-010 STOP solution 14 ml (Ready-to-use) 4°C

Overview

Product Description ARG80929 Human Inflammatory Cytokine Multiplex ELISA Kit (IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha) is an Enzyme Immunoassay kit for the semi-quantification of IL1 alpha, IL1 beta, IL6, IL8, GM-CSF, IFN-gamma, MCAF and TNF-alpha in serum, plasma, cell culture supernatant and other biological samples.

See all Multiplex ELISA kits
Tested Reactivity Hu
Tested Application ELISA
Target Name Inflammatory Cytokine Multiplex
Conjugation HRP
Conjugation Note

At a wavelength of 450 nm

Detection Range IL1 alpha: 20.32 - 650 pg/ml
IL1 beta: 23.44 - 750 pg/ml
IL6: 7.82 - 250 pg/ml
IL8: 25 - 800 pg/ml
GM-CSF: 17.19 - 550 pg/ml
IFN-gamma: 10.94 - 350 pg/ml
MCAF: 25 - 800 pg/ml
TNF-alpha: 17.19 - 550 pg/ml
Sample Type Serum, plasma, cell culture supernatant and other biological samples.
Sample Volume 100 µl

Application Instructions

Assay Time 3.5 hour

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Inflammation is body's protective response to foreign subjects, pathogens, tissue damage, autoimmune and other harmful stimuli. It is initiated by production of a cascade of chemicals and cytokines in affected area. These pro-inflammatory mediators result in vasodilation, increased vascular permeability, influx of blood, plasma leakage, neutrophil and macrophage infiltration and activation. Inflammatory reaction plays an important role in limiting foreign substance and engulfing pathogens and tissue debris.

Acute inflammation is the initial phase to eliminate invaded foreign substances, pathogens and other harmful stimuli. If the stimuli persist, chronic inflammation will evolve. Progressive tissue destruction and shifting cell types occurs simultaneously during chronic inflammation. The cytokine profiles that regulate the procedure are different depending on the causes, location and progress of the inflammation.
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Research Area Immune System kit; Metabolism kit

Specific References

Long-Term Changes in Cytokine and Ghrelin Levels Following Laparoscopic Sleeve Gastrectomy in Obese Individuals

ELISA / Human / Tissue lysate

Spyridon Spanakos et al.
World J Surg Surg Res.,  (2022)

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Antimicrobial and Immunomodulatory Activity of Herb Extracts Used in Burn Wound Healing: "San Huang Powder"

ELISA / Human / Cell culture supernatants

Jia-Ru Wu et al.
Evid Based Complement Alternat Med.,  (2021)

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Title Download Link
ARG80929 Human Inflammatory Cytokine multiplex ELISA Kit User manual download icon Download