ARG82313
Human Insulin ELISA Kit
Human Insulin ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82313 Human Insulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Insulin in serum and plasma (heparin, EDTA). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: Insulin: 100% Glargine: 104% Aspart: 94% Lispro: 108% Proinsulin: 1.2% C-peptide: 0% |
| Target Name | Insulin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.9 mU/l |
| Sample Type | Serum and plasma (heparin, EDTA). |
| Standard Range | 8.7 - 245 mU/l |
| Sample Volume | 25 µl |
| Precision | CV: 6.5% |
| Alternate Names | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN |
Application Instructions
| Assay Time | ~ 2.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | INS |
| Gene Full Name | insulin |
| Background | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Insulin antibodies; Insulin ELISA Kits; Insulin Duos / Panels; Insulin recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
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| ARG82313 Human Insulin ELISA Kit User manual |
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