ARG70323

Human Irisin recombinant protein (Active) (His-tagged, C-ter)

Human Irisin recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Active Human Irisin recombinant protein.
Tested Reactivity Hu
Tested Application FuncSt, SDS-PAGE
Target Name Irisin
Species Human
A.A. Sequence Asp32 - Glu143 of Human Irisin (NP_715637.2) with 6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured by its ability to induce p38 MAPK activation in 3T3-L1 mouse embryonic fibroblast adipose-like cells. 0.1 µg/ml of Recombinant Human Irisin can effectively induce p38 MAPK activation.
Alternate Names irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 252995 Human FNDC5

Swiss-port # Q8NAU1 Human Fibronectin type III domain-containing protein 5

Gene Symbol FNDC5
Gene Full Name fibronectin type III domain containing 5
Background This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Function [Irisin]: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein. Note=Imported in peroxisomes through the PEX5 receptor pathway. Irisin: Secreted. Note=Detected in the blood of individuals subjected to endurance exercise. [UniProt]
Calculated MW 24 kDa
PTM The extracellular domain is cleaved and released from the cell membrane.

N-Glycosylated. [UniProt]