ARG70323
Human Irisin recombinant protein (Active) (His-tagged, C-ter)
Human Irisin recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Active Human Irisin recombinant protein. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt, SDS-PAGE |
| Target Name | Irisin |
| Species | Human |
| A.A. Sequence | Asp32 - Glu143 of Human Irisin (NP_715637.2) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Measured by its ability to induce p38 MAPK activation in 3T3-L1 mouse embryonic fibroblast adipose-like cells. 0.1 µg/ml of Recombinant Human Irisin can effectively induce p38 MAPK activation. |
| Alternate Names | irisin; FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2 |
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8NAU1 Human Fibronectin type III domain-containing protein 5 |
|---|---|
| Gene Symbol | FNDC5 |
| Gene Full Name | fibronectin type III domain containing 5 |
| Background | This gene encodes a secreted protein that is released from muscle cells during exercise. The encoded protein may participate in the development of brown fat. Translation of the precursor protein initiates at a non-AUG start codon at a position that is conserved as an AUG start codon in other organisms. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| Function | [Irisin]: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Peroxisome membrane; Single-pass type I membrane protein. Note=Imported in peroxisomes through the PEX5 receptor pathway. Irisin: Secreted. Note=Detected in the blood of individuals subjected to endurance exercise. [UniProt] |
| Calculated MW | 24 kDa |
| PTM | The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated. [UniProt] |
