ARG82403

Human JAM-C ELISA Kit

Human JAM-C ELISA Kit for ELISA and Human

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Component

Cat No Component Name Package Temp
ARG82403-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82403-002 Standard 2 X 10 ng/vial 4°C
ARG82403-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82403-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82403-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82403-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82403-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82403-008 25X Wash buffer 20 ml 4°C
ARG82403-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82403-010 STOP solution 10 ml (Ready to use) 4°C
ARG82403-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82403 Human JAM-C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human JAM-C in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name JAM-C
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 23.45 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 46.9 - 3000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.0%
Inter-Assay CV: 6.0%
Alternate Names JAM-2; JAM-3; Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAMC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 83700 Human JAM3

Swiss-port # Q9BX67 Human Junctional adhesion molecule C

Gene Symbol JAM3
Gene Full Name junctional adhesion molecule 3
Background Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
Function Participates in cell-cell adhesion. It is a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). The soluble form is a mediator of angiogenesis. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome. Secreted, extracellular space. Note=In epithelial cells, it is expressed at desmosomes but not at tight junctions (PubMed:15194813). Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitment of JAM3 to cell-cell contacts (PubMed:15994945). [UniProt]
Highlight Related products:
JAM-C antibodies; JAM-C ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors. [UniProt]

Specific References

Plasma junctional adhesion molecule C levels are associated with the presence and severity of coronary artery disease.

ELISA / Human

Zhenguo Wu et al.
Clin Biochem.,  (2023)

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ARG82403 Human JAM-C ELISA Kit User manual download icon Download