ARG82380
Human JAML ELISA Kit
Human JAML ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82380-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82380-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82380-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82380-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82380-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82380-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82380-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82380-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82380-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82380-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82380-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82380 Human JAML ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human JAML in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | JAML |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 6.8% |
| Alternate Names | Junctional adhesion molecule-like; Gm638; Dendritic cell-specific protein CREA7-1; CREA7-1; CREA7-4; Adhesion molecule interacting with CXADR antigen 1; JAML; AMICA |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AMICA1 |
| Gene Full Name | adhesion molecule, interacts with CXADR antigen 1 |
| Function | Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell junction. Note=Localized at the plasma membrane and enriched in areas of cell-cell contacts (PubMed:12869515). [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82380 Human JAML ELISA Kit User manual |
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